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BillionToOne Valuation Multiples
Discover revenue and EBITDA valuation multiples for BillionToOne and similar public comparables like Hamamatsu Photonics, LivaNova, ICU Medical, Sectra and more.
BillionToOne Overview
About BillionToOne
BillionToOne Inc is a precision diagnostics company revolutionizing molecular testing with its patented Quantitative Counting Templates (QCT) platform. This technology enables ultrasensitive, single-molecule DNA quantification, making genetic testing more accurate, affordable, and accessible. The company serves prenatal screening and oncology markets with non-invasive tests that detect fetal genetic risks and monitor cancer therapy response.
Founded
2016
HQ

Employees
N/A
Website
Financials (LTM)
EV
$4B
BillionToOne Financials
BillionToOne reported last 12-month revenue of $340M and EBITDA of $41M.
In the same LTM period, BillionToOne generated $233M in gross profit, $41M in EBITDA, and $9M in net income.
Revenue (LTM)
BillionToOne P&L
In the most recent fiscal year, BillionToOne reported revenue of $305M and EBITDA of $37M.
BillionToOne expects next 12-month revenue of XXX and NTM EBITDA of XXX
| LTM | NTM | Last FY | FY 2026 | FY 2027 | FY 2028 | |
|---|---|---|---|---|---|---|
| Revenue | $340M | XXX | $305M | XXX | XXX | XXX |
| Gross Profit | $233M | XXX | $208M | XXX | XXX | XXX |
| Gross Margin | 69% | XXX | 68% | XXX | XXX | XXX |
| EBITDA | $41M | XXX | $37M | XXX | XXX | XXX |
| EBITDA Margin | 12% | XXX | 12% | XXX | XXX | XXX |
| EBIT Margin | 5% | XXX | 5% | XXX | XXX | XXX |
| Net Profit | $9M | XXX | $7M | XXX | XXX | XXX |
| Net Margin | 3% | XXX | 2% | XXX | XXX | XXX |
Financial data powered by Morningstar, Inc.
BillionToOne Stock Performance
BillionToOne has current market cap of $4B, and enterprise value of $4B.
Market Cap Evolution
BillionToOne's stock price is $80.83.
| EV | Market Cap | Price 1D | Price 1M | Price 3M | Price 12M | EPS |
|---|---|---|---|---|---|---|
| $4B | $4B | 0.0% | XXX | XXX | XXX | $0.16 |
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Start Free TrialBillionToOne Valuation Multiples
BillionToOne trades at 10.6x EV/Revenue multiple, and 89.0x EV/EBITDA.
EV / Revenue (LTM)
BillionToOne Financial Valuation Multiples
As of April 8, 2026, BillionToOne has market cap of $4B and EV of $4B.
Equity research analysts estimate BillionToOne's 2025E EV/Revenue multiple at XXX and 2025E EV/EBITDA multiple at XXX
BillionToOne has a P/E ratio of 403.1x.
| LTM | NTM | Last FY | FY 2026 | FY 2027 | FY 2028 | |
|---|---|---|---|---|---|---|
| Market cap (current) | $4B | XXX | $4B | XXX | XXX | XXX |
| EV (current) | $4B | XXX | $4B | XXX | XXX | XXX |
| EV/Revenue | 10.6x | XXX | 11.8x | XXX | XXX | XXX |
| EV/EBITDA | 89.0x | XXX | 96.8x | XXX | XXX | XXX |
| EV/EBIT | 197.8x | XXX | 225.5x | XXX | XXX | XXX |
| EV/Gross Profit | 15.5x | XXX | 17.3x | XXX | XXX | XXX |
| P/E | 403.1x | XXX | 496.3x | XXX | XXX | XXX |
| EV/FCF | 241.9x | XXX | 230.5x | XXX | XXX | XXX |
Valuation data powered by FactSet, Inc. and Morningstar, Inc.
Verified BillionToOne Valuation Multiples
Access all public comps and forward-looking valuation multiples like EV/Revenue in 2027, based on consensus analyst estimates. Powered by FactSet and Morningstar.


BillionToOne Margins & Growth Rates
BillionToOne's revenue in the last 12 month grew by 39%.
BillionToOne's rule of 40 is 54% (metric relevant for SaaS companies only, counted as combined revenue growth rate and EBITDA margin).
BillionToOne's rule of X is 119% (created by Bessemer, rule of X is another metric to measure SaaS companies, ~1.5x stronger vs. the traditional Rule of 40, counted as revenue growth rate multiplied by 2.5 plus EBITDA margin).
BillionToOne Operational Valuation Multiples
| LTM | NTM | Last FY | FY 2026 | FY 2027 | FY 2028 | |
|---|---|---|---|---|---|---|
| Revenue Growth | 39% | XXX | 43% | XXX | XXX | XXX |
| EBITDA Margin | 12% | XXX | 12% | XXX | XXX | XXX |
| EBITDA Growth | 47% | XXX | 33% | XXX | XXX | XXX |
| Rule of 40 | — | XXX | 54% | XXX | XXX | XXX |
| Bessemer Rule of X | — | XXX | 119% | XXX | XXX | XXX |
| S&M Expenses to Revenue | 32% | XXX | 33% | XXX | XXX | XXX |
| G&A Expenses to Revenue | 15% | XXX | 14% | XXX | XXX | XXX |
| R&D Expenses to Revenue | 17% | XXX | 16% | XXX | XXX | XXX |
| Opex to Revenue | — | XXX | 63% | XXX | XXX | XXX |
Valuation data powered by FactSet, Inc. and Morningstar, Inc.
BillionToOne Public Comps
See public comps and valuation multiples for other Diagnostics & Genomics and Medical Devices comps.
| EV/Revenue | EV/EBITDA | |||||
|---|---|---|---|---|---|---|
| LTM | 2026E | 2027E | LTM | 2026E | 2027E | |
| Hamamatsu Photonics | XXX | XXX | XXX | XXX | XXX | XXX |
| LivaNova | XXX | XXX | XXX | XXX | XXX | XXX |
| ICU Medical | XXX | XXX | XXX | XXX | XXX | XXX |
| Sectra | XXX | XXX | XXX | XXX | XXX | XXX |
| MGI Tech | XXX | XXX | XXX | XXX | XXX | XXX |
| XXXXXXXX | XXX | XXX | XXX | XXX | XXX | XXX |
| XXXXXXXX | XXX | XXX | XXX | XXX | XXX | XXX |
Valuation data powered by FactSet, Inc.
BillionToOne M&A Activity
BillionToOne acquired XXX companies to date.
Last acquisition by BillionToOne was on XXXXXXXX, XXXXX. BillionToOne acquired XXXXXXXX for XXX (EV/Revenue multiple of XXX).
Latest Acquisitions by BillionToOne
| Acquired Company | Valuation | EV/Revenue | EV/EBITDA |
|---|---|---|---|
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
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Start Free TrialBillionToOne Investment Activity
BillionToOne invested in XXX companies to date.
BillionToOne made its latest investment on XXXXXXXX, XXXXX. BillionToOne invested in XXXXXXXX for XXX (EV/Revenue multiple of XXX).
Latest Investments by BillionToOne
| Company | Valuation | EV/Revenue | EV/EBITDA |
|---|---|---|---|
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
| XXXXXXXXXXXX | XXX | XXX | XXX |
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Start Free TrialAbout BillionToOne
| When was BillionToOne founded? | BillionToOne was founded in 2016. |
| Where is BillionToOne headquartered? | BillionToOne is headquartered in United States. |
| Is BillionToOne publicly listed? | Yes, BillionToOne is a public company listed on Nasdaq. |
| What is the stock symbol of BillionToOne? | BillionToOne trades under BLLN ticker. |
| When did BillionToOne go public? | BillionToOne went public in 2025. |
| Who are competitors of BillionToOne? | BillionToOne main competitors are Hamamatsu Photonics, LivaNova, ICU Medical, Sectra. |
| What is the current market cap of BillionToOne? | BillionToOne's current market cap is $4B. |
| What is the current revenue of BillionToOne? | BillionToOne's last 12 months revenue is $340M. |
| What is the current revenue growth of BillionToOne? | BillionToOne revenue growth (NTM/LTM) is 39%. |
| What is the current EV/Revenue multiple of BillionToOne? | Current revenue multiple of BillionToOne is 10.6x. |
| Is BillionToOne profitable? | Yes, BillionToOne is EBITDA-positive (as of the last 12 months). |
| What is the current EBITDA of BillionToOne? | BillionToOne's last 12 months EBITDA is $41M. |
| What is BillionToOne's EBITDA margin? | BillionToOne's last 12 months EBITDA margin is 12%. |
| What is the current EV/EBITDA multiple of BillionToOne? | Current EBITDA multiple of BillionToOne is 89.0x. |
| What is the current FCF of BillionToOne? | BillionToOne's last 12 months FCF is $15M. |
| What is BillionToOne's FCF margin? | BillionToOne's last 12 months FCF margin is 4%. |
| What is the current EV/FCF multiple of BillionToOne? | Current FCF multiple of BillionToOne is 241.9x. |
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