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BillionToOne Valuation Multiples

Discover revenue and EBITDA valuation multiples for BillionToOne and similar public comparables like 4basebio, ToolGen, and Mirxes Holding Co.

BillionToOne Overview

About BillionToOne

BillionToOne Inc is a precision diagnostics company revolutionizing molecular testing with its patented Quantitative Counting Templates (QCT) platform. This technology enables ultrasensitive, single-molecule DNA quantification, making genetic testing more accurate, affordable, and accessible. The company serves prenatal screening and oncology markets with non-invasive tests that detect fetal genetic risks and monitor cancer therapy response.


Founded

2016

HQ

United States of America
Employees

n/a

Financials

LTM Revenue $296M

LTM EBITDA $23.0M

EV

$4.0B

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BillionToOne Financials

As of January 2026, BillionToOne reported last 12-month revenue of $296M and EBITDA of $23.0M.

In the same period, BillionToOne generated $198M in LTM gross profit and $2.2M in net income.

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BillionToOne P&L

In the most recent fiscal year, BillionToOne reported revenue of $153M and EBITDA of -$27.5M.

BillionToOne expects next 12-month revenue of  XXX    and NTM EBITDA of  XXX

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LTM NTM Last FY FY 2026 FY 2027 FY 2028
Revenue $296M XXX $153M XXX XXX XXX
Gross Profit $198M XXX $80.9M XXX XXX XXX
Gross Margin 67% XXX 53% XXX XXX XXX
EBITDA $23.0M XXX -$27.5M XXX XXX XXX
EBITDA Margin 8% XXX -18% XXX XXX XXX
EBIT $8.2M XXX -$47.1M XXX XXX XXX
EBIT Margin 3% XXX -31% XXX XXX XXX
Net Profit $2.2M XXX -$41.6M XXX XXX XXX
Net Margin 1% XXX -27% XXX XXX XXX
Net Debt XXX XXX n/a XXX XXX XXX

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BillionToOne Stock Performance

BillionToOne has current market cap of $4.1B, and EV of $4.0B.

Market Cap Evolution

BillionToOne Stock Data

As of February 20, 2026, BillionToOne's stock price is $89.

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EV Market Cap Price 1D Price 1M Price 3M Price 12M EPS
$4.0B $4.1B XXX XXX XXX XXX $0.10

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BillionToOne Valuation Multiples

BillionToOne's trades at 13.5x EV/Revenue multiple, and 173.3x EV/EBITDA.

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BillionToOne Financial Valuation Multiples

As of February 20, 2026, BillionToOne has market cap of $4.1B and EV of $4.0B.

Equity research analysts estimate BillionToOne's 2025E EV/Revenue multiple at  XXX and 2025E EV/EBITDA multiple at  XXX

BillionToOne has a P/E ratio of 1884.9x.

LTM NTM Last FY FY 2026 FY 2027 FY 2028
Market cap (current) $4.1B XXX $4.1B XXX XXX XXX
EV (current) $4.0B XXX $4.0B XXX XXX XXX
EV/Revenue 13.5x XXX 13.5x XXX XXX XXX
EV/EBITDA 173.3x XXX 173.3x XXX XXX XXX
EV/EBIT 485.5x XXX 485.5x XXX XXX XXX
EV/Gross Profit 20.1x XXX n/a XXX XXX XXX
P/E 1884.9x XXX -98.1x XXX XXX XXX
EV/FCF 9979.7x XXX 9979.7x XXX XXX XXX

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BillionToOne Margins & Growth Rates

BillionToOne's last 12 month revenue growth is 36%

BillionToOne's revenue per employee in the last FY averaged n/a, while opex per employee averaged n/a for the same period.

BillionToOne's rule of 40 is -66% (metric relevant for SaaS companies only, counted as combined revenue growth rate and EBITDA margin).

BillionToOne's rule of X is 97% (created by Bessemer, rule of X is another metric relevant for SaaS companies only, ~1.5x stronger vs. the traditional Rule of 40, counted as revenue growth rate multiplied by 2.5 plus EBITDA margin).

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BillionToOne Operational Valuation Multiples

LTM NTM Last FY FY 2026 FY 2027 FY 2028
Revenue Growth 36% XXX 35% XXX XXX XXX
EBITDA Margin 8% XXX 8% XXX XXX XXX
EBITDA Growth 31% XXX n/a XXX XXX XXX
Rule of 40 -66% XXX 44% XXX XXX XXX
Bessemer Rule of X XXX XXX 97% XXX XXX XXX
Revenue per Employee XXX XXX n/a XXX XXX XXX
Opex per Employee XXX XXX n/a XXX XXX XXX
S&M Expenses to Revenue XXX XXX n/a XXX XXX XXX
G&A Expenses to Revenue XXX XXX n/a XXX XXX XXX
R&D Expenses to Revenue XXX XXX 24% XXX XXX XXX
Opex to Revenue XXX XXX 84% XXX XXX XXX

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Valuation Multiples Across 220+ Verticals

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BillionToOne Public Comps

See public comps and valuation multiples for Genomics & Personalized Medicine comps
EV/Revenue EV/EBITDA
2025E 2026E 2027E 2025E 2026E 2027E
EZZ Life Science XXX XXX XXX XXX XXX XXX
Epigenomics XXX XXX XXX XXX XXX XXX
Mirxes Holding Co XXX XXX XXX XXX XXX XXX
ToolGen XXX XXX XXX XXX XXX XXX
4basebio XXX XXX XXX XXX XXX XXX
XXXXXXXX XXX XXX XXX XXX XXX XXX
XXXXXXXX XXX XXX XXX XXX XXX XXX

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BillionToOne M&A and Investment Activity

BillionToOne acquired  XXX companies to date.

Last acquisition by BillionToOne was  XXXXXXXX, XXXXX XXXXX XXXXXX . BillionToOne acquired  XXXXXXXX for  XXX (EV/Revenue multiple of  XXX ).

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Latest Acquisitions by BillionToOne

Acquired Company EV EV/Revenue EV/EBITDA
XXXXXXXXX XXX XXX XXX
XXXXXXXXX XXX XXX XXX
XXXXXXXXX XXX XXX XXX
XXXXXXXXX XXX XXX XXX
XXXXXXXXX XXX XXX XXX

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About BillionToOne

When was BillionToOne founded? BillionToOne was founded in 2016.
Where is BillionToOne headquartered? BillionToOne is headquartered in United States of America.
Who is the CEO of BillionToOne? BillionToOne's CEO is Dr. Oguzhan Atay, PhD.
Is BillionToOne publicy listed? Yes, BillionToOne is a public company listed on NAS.
What is the stock symbol of BillionToOne? BillionToOne trades under BLLN ticker.
When did BillionToOne go public? BillionToOne went public in 2025.
Who are competitors of BillionToOne? Similar companies to BillionToOne include e.g. EZZ Life Science, Epigenomics, Mirxes Holding Co, ToolGen.
What is the current market cap of BillionToOne? BillionToOne's current market cap is $4.1B
What is the current revenue of BillionToOne? BillionToOne's last 12 months revenue is $296M.
What is the current revenue growth of BillionToOne? BillionToOne revenue growth (NTM/LTM) is 36%.
What is the current EV/Revenue multiple of BillionToOne? Current revenue multiple of BillionToOne is 13.5x.
Is BillionToOne profitable? Yes, BillionToOne is EBITDA-positive (as of the last 12 months).
What is the current EBITDA of BillionToOne? BillionToOne's last 12 months EBITDA is $23.0M.
What is BillionToOne's EBITDA margin? BillionToOne's last 12 months EBITDA margin is 8%.
What is the current EV/EBITDA multiple of BillionToOne? Current EBITDA multiple of BillionToOne is 173.3x.
What is the current FCF of BillionToOne? BillionToOne's last 12 months FCF is $0.4M.
What is BillionToOne's FCF margin? BillionToOne's last 12 months FCF margin is 0%.
What is the current EV/FCF multiple of BillionToOne? Current FCF multiple of BillionToOne is 9979.7x.

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